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Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules

Indian Institute of Science Bangalore via NPTEL

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Overview

The objective of the course is to introduce methods used in NMR spectroscopy for structure determination of biomolecules and for studying protein-ligand interactions. The course will cover principles and application of two- and three-dimensional NMR experiments along with different isotope labelling schemes that are routinely used for protein structure determination.

INTENDED AUDIENCE:
M. Sc/Ph. D students, Scientists from Pharma/Biotech Companies

PREREQUISITES: 1. Mathematics at the 12th Std level,2. Basic Knowledge of Proteins/Nucleic Acids,3. A basic knowledge of NMR would be useful,though not necessary

Syllabus

Week 1  :  Introduction to Basics of NMR
Week 2  :  Two dimensional NMR
Week 3  :  Important 2D NMR experiments for Biomolecules
Week 4  :  3D NMR Spectroscopy
Week 5  :  Basics of Protein and Nucleic Acid Structure
Week 6  :  Isotope Labelling
Week 7  :  Resonance assignment of Proteins with NMR and Structure Determination
Week 8  :  NMR Experiments for Studying Protein-Ligand Interactions

Taught by

Prof. Hanudatta S. Atreya

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