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Intro
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Classroom Contents
Fluorescence in One Hour
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- 1 Intro
- 2 Electromagnetic spectrum
- 3 What happens? Example: ketone
- 4 Molecular spectroscopy
- 5 Principles of spectroscopy
- 6 Principles of fluorescence
- 7 Tryptophan fluorescence
- 8 Fluorescence spectroscopy
- 9 Internal relaxation
- 10 Fluorescence dictionary - Part 11
- 11 Varian Eclipse
- 12 Xenon flash lamp
- 13 Instrumentation - PMT detector
- 14 Fluorophores - Molecular structure
- 15 Flourophores
- 16 Factors affecting the fluorescence signal
- 17 Concentration - Ideal conditions
- 18 Inner filter effect
- 19 Problem with the correction
- 20 Environment - Solvent
- 21 Environment - Temperature
- 22 Environment - Denaturant
- 23 Dynamic quenching
- 24 Static quenching
- 25 Non-radiative energy transfer
- 26 Scatter
- 27 Ways to measure fluorescence - Polarization
- 28 Ways to measure fluorescence - Time-decay
- 29 Fluorescence summary
- 30 Why fluorescence?
- 31 Options of measuring fluorescence
- 32 Second Order Advantage - PLS VS. PARAFAC
- 33 Proteins and salt solutions
