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Intro
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Classroom Contents
Illumina Sequencing Overview - Library Prep to Data Analysis
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- 1 Intro
- 2 Session Objectives
- 3 Illumina Sequencing Workflow
- 4 Library Prep is Critical for Successful Sequencing
- 5 What is a Cluster?
- 6 What is a flow cell?
- 7 Flow cell Architecture Random vs Pattemed
- 8 Patterned flow cells Complete control of pitch & feature size
- 9 Hybridize Fragment & Extend
- 10 Denature Double-Stranded DNA
- 11 Single-Stranded DNA
- 12 Bridge Amplification
- 13 Denature Double-Stranded Bridge
- 14 Linearization
- 15 Reverse Strand Cleavage
- 16 Read 1 Primer Hybridization
- 17 Patterned Flow Cell and ExAmp Technology
- 18 Illumina Sequencing Systems
- 19 Dye Chemistry 4-channel chemistry
- 20 2-Channel SBS Chemistry
- 21 Dye Chemistry 2-channel chemistry
- 22 Sequencing by synthesis with CMOS detection 1-channel chemistry
- 23 Illumina Chemistry Comparison
- 24 Paired-End Sequencing
- 25 Single Index Reads
- 26 Dual Index Reads
- 27 Analysis Overview
