Integrate Single-Cell RNA-Seq Datasets in R Using Seurat - Detailed Seurat Workflow Tutorial

Integrate Single-Cell RNA-Seq Datasets in R Using Seurat - Detailed Seurat Workflow Tutorial

bioinformagician via YouTube Direct link

Intro

1 of 14

1 of 14

Intro

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Integrate Single-Cell RNA-Seq Datasets in R Using Seurat - Detailed Seurat Workflow Tutorial

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  1. 1 Intro
  2. 2 Study design
  3. 3 When to integrate?
  4. 4 Types of integration
  5. 5 Batch correction methods
  6. 6 Downloading data
  7. 7 Read data in R
  8. 8 Merge Seurat objects
  9. 9 QC and filtering
  10. 10 Do we see batch effects in our data?
  11. 11 Visualize merged data before integration
  12. 12 Integration steps
  13. 13 Visualize integrated data after integration
  14. 14 Comparing UMAPs: before integration vs after integration

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