This course on eukaryotic molecular biology aims to teach the structures and functions of DNA and histone molecules, eukaryotic genome organization, transposon mechanisms, eukaryotic transcription processes, RNA biology including splicing and polyadenylation. The course covers skills such as understanding nucleosome structure, histone modification, transposon action, and RNA processing. The teaching method includes video lectures. The intended audience for this course includes students and professionals interested in molecular biology and genetics.
Overview
Syllabus
Higher order nucleosome structure.
Nucleosome and histones | Nucleosome structure.
Histone acetylation | histone acetyltransferase.
Nucleosome modification.
Nucleosome Remodeling.
Genetic organization of LINE and SINE.
Activator transposable elements.
Different mechanisms of transposition.
Mechanism of transposition of retroviral transposons.
Composite and noncomposite transposons.
Cut and paste DNA transposition.
Transposition of a poly A retrotransposon.
Transposition of IS elements.
IS elements.
Ds and Ac transposable elements.
Eukaryotic transcription part 1: introduction.
Eukaryotic transcription part 2: Polymerase I mediated transcription.
Eukaryotic transcrption part 3: polymerase II mediated transcription.
Eukaryotic transcription part 4: Polymerase III mediated transcription.
Eukaryotic transcription part 5: eukaryotic transcription summary and termination.
RNA splicing part 1 (introduction).
RNA splicing part 2 (self splicing).
RNA splicing part 3 (spliceosome mediated splicing).
RNA splicing part 4 (classification).
Protein splicing.
RNA modifications (synthesis of 5 prime cap).
RNA modifications: 5 prime cap.
Polyadenylation of mRNA (poly a tail).
RNA editing: adenosine to inosine.
RNA editing introduction.
Guide RNA dependent RNA editing.
RNA editing mechanism.
RNA purification.
Chromatin remodeling.
DNA methylation.
Taught by
Shomu"s Biology
